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{
    "id": 144,
    "name": "Reference-based RNA-Seq data analysis with Galaxy",
    "description": "This tutorial covers the questions:\r\n- What are the steps to process RNA-Seq data?\r\n- How to identify differentially expressed genes across multiple experimental conditions?\r\n- What are the biological functions impacted by the differential expression of genes?\r\n\r\nAt the end of the tutorial, learners would be able to:\r\n- Check a sequence quality report generated by FastQC for RNA-Seq data\r\n- Explain the principle and specificity of mapping of RNA-Seq data to an eukaryotic reference genome\r\n- Select and run a state of the art mapping tool for RNA-Seq data\r\n- Evaluate the quality of mapping results\r\n- Describe the process to estimate the library strandness\r\n- Estimate the number of reads per genes\r\n- Explain the count normalization to perform before sample comparison\r\n- Construct and run a differential gene expression analysis\r\n- Analyze the DESeq2 output to identify, annotate and visualize differentially expressed genes\r\n- Perform a gene ontology enrichment analysis\r\n- Perform and visualize an enrichment analysis for KEGG pathways",
    "communities": [],
    "elixirPlatforms": [],
    "doi": null,
    "fileLocation": "https://training.galaxyproject.org/training-material/topics/transcriptomics/tutorials/ref-based/tutorial.html",
    "fileName": "rna-seq",
    "topics": [
        "http://edamontology.org/topic_0102",
        "http://edamontology.org/topic_1775",
        "http://edamontology.org/topic_0203",
        "http://edamontology.org/topic_3308",
        "http://edamontology.org/topic_3170"
    ],
    "keywords": [
        "Galaxy",
        "RNA-seq"
    ],
    "audienceTypes": [
        "Undergraduate",
        "Graduate",
        "Professional (initial)",
        "Professional (continued)"
    ],
    "audienceRoles": [
        "Researchers",
        "Life scientists",
        "Biologists"
    ],
    "difficultyLevel": "Novice",
    "providedBy": [],
    "dateCreation": null,
    "dateUpdate": null,
    "licence": "CC-BY-4.0",
    "maintainers": [
        "https://catalogue.france-bioinformatique.fr/api/userprofile/677/?format=api"
    ]
}