This course offers an introduction to metabarcoding analyses at two different levels/steps: bioinformatics with FROGS pipeline in the Galaxy environment, biostatistics with PhyloSeq R package. This includes preprocessing, clustering and OTU picking, taxonomic assignation, estimation of diversity, visualization of statistics results. Prerequisites Galaxy, R knowledge
Program Introduction to metagenomics and metabarcoding Pre-processing, Clustering, taxonomic affiliation (FROGS) Handling and visualizing OTU table using PhyloSeq R package (PhyloSeq)
Learning objectives Manipulate tools available for metabarcoding analysis Study sample diversity by using NGS and post-NGS analysis tools Visualize diversity metrics in metabarcoding approach