{"id":388,"name":"RNASeq analyses (using Galaxy and TOGGLe)","shortName":"","description":"This course offers an introduction to RNASeq analyses using two different workflow management systems: Galaxy and TOGGLe. This includes reference-based mapping, estimates of transcript levels, differential expression (DE) analyses, visualization of statistics results.\nPrerequisites\nWorkflow management system (Galaxy, TOGGLe)\n\nProgram\nMapping of RNASeq against a transcriptome reference with kallisto (Galaxy)\nMapping of RNASeq against an annotated genome reference with TopHat (TOGGLe)\nDifferential expression analysis using EdgeR and DESeq2\nPlots, clustering, co-expression network: degust, WGCNA\n\n\nLearning objectives\nManipulate packages/tools available for searching DE genes\nThink about different normalisation methods\nDetect differentially expressed genes\nCompare results between two approaches\n\n\nInstructors\nAlexis Dereeper - alexis.dereeper@ird.fr\nSebastien Cunnac - sebastien.cunnac@ird.fr\nSebastien Ravel - sebastien.ravel@cirad.fr\nChristine Tranchant  - christine.tranchant@ird.fr\n\n","homepage":"https://southgreenplatform.github.io/trainings//rnaseq/","is_draft":false,"costs":["Free"],"topics":[],"keywords":[],"prerequisites":[],"openTo":"Internal personnel","accessConditions":"","maxParticipants":null,"contacts":[],"elixirPlatforms":[],"communities":[],"sponsoredBy":[],"organisedByOrganisations":[],"organisedByTeams":[{"id":24,"name":"South Green","url":"https://catalogue.france-bioinformatique.fr/api/team/South%20Green/?format=json"}],"logo_url":"","updated_at":"2022-06-02T11:50:50.627601Z","type":"Training course","start_date":"2018-03-15","end_date":null,"venue":"","city":"Centre IRD, Montpellier","country":"","geographical_range":"","trainers":[],"trainingMaterials":[],"computingFacilities":[],"realisation_status":"past","registration_opening":null,"registration_closing":null,"registration_status":"unknown","courseMode":null}